Ioerger TR, Koo S, No EG, Chen X, Larsen MH, Jacobs WR Jr, Pillay M, Sturm AW, Sacchettini JC. "Genome analysis of multi- and extensively-drug-resistant tuberculosis from KwaZulu-Natal, South Africa." PLoS One. 2009 Nov 5;4(11):e7778.
Sun Q, Kuty GF, Arockiasamy A, Xu M, Young R, Sacchettini JC. "Regulation of a muralytic enzyme by dynamic membrane topology." Nat Struct Mol Biol. 2009 Nov;16(11):1192-4. Epub 2009 Nov 1.
Reddy MC, Palaninathan SK, Bruning JB, Thurman C, Smith D, Sacchettini JC. "Structural insights into the mechanism of the allosteric transitions of the mycobacterium tuberculosis cAMP receptor protein." J Biol Chem. 2009 Dec 25;284(52):36581-91.
Palaninathan SK, Mohamedmohaideen NN, Orlandini E, Ortore G, Nencetti S, Lapucci A, Rossello A, Freundlich JS, Sacchettini JC. "Novel transthyretin amyloid fibril formation inhibitors: synthesis, biological evaluation, and X-ray structural analysis." PLoS One. 2009 Jul 21;4(7):e6290.
Swanson S, Gokulan K, Sacchettini JC. "KasA, another brick in the mycobacterial cell wall." Structure. 2009 Jul 15;17(7):914-5.
Ioerger TR, Sacchettini JC. "Structural genomics approach to drug discovery for Mycobacterium tuberculosis." Curr Opin Microbiol. 2009 Jun;12(3):318-25. Epub 2009 May 28. Review.
Barkan D, Liu Z, Sacchettini JC, Glickman MS. "Mycolic acid cyclopropanation is essential for viability, drug resistance, and cell wall integrity of Mycobacterium tuberculosis." Chem Biol. 2009 May 29;16(5):499-509.
Freundlich JS, Wang F, Vilchèze C, Gulten G, Langley R, Schiehser GA, Jacobus DP, Jacobs WR Jr, Sacchettini JC. "Triclosan derivatives: towards potent inhibitors of drug-sensitive and drug-resistant Mycobacterium tuberculosis." ChemMedChem. 2009 Feb;4(2):241-8.
Publications 2008
Yu M, Kumar TR, Nkrumah LJ, Coppi A, Retzlaff S, Li CD, Kelly BJ, Moura PA, Lakshmanan V, Freundlich JS, Valderramos JC, Vilcheze C, Siedner M, Tsai JH, Falkard B, Sidhu AB, Purcell LA, Gratraud P, Kremer L, Waters AP, Schiehser G, Jacobus DP, Janse CJ, Ager A, Jacobs WR Jr, Sacchettini JC, Heussler V, Sinnis P, Fidock DA. "The fatty acid biosynthesis enzyme FabI plays a key role in the development of liver-stage malarial parasites" Cell Host Microbe. 2008 Dec 11;4(6):567-78.
Reddy MC, Kuppan G, Shetty ND, Owen JL, Ioerger TR, Sacchettini JC. "Crystal structures of Mycobacterium tuberculosis S-adenosyl-L-homocysteine hydrolase in ternary complex with substrate and inhibitors" Protein Sci. 2008 Dec;17(12):2134-44.
Cho Y, Ioerger TR, Sacchettini JC. "Discovery of Novel Nitrobenzothiazole Inhibitors for Mycobacterium tuberculosis ATP Phosphoribosyl Transferase (HisG) through Virtual Screening." J Med Chem. 2008 Sep 9.
Palaninathan SK, Mohamedmohaideen NN, Snee WC, Kelly JW, Sacchettini JC. "Structural Insight into pH-Induced Conformational Changes within the Native Human Transthyretin Tetramer." J Mol Biol. 2008 Jul 16.
Vilchèze C, Av-Gay Y, Attarian R, Liu Z, Hazbón MH, Colangeli R, Chen B, Liu W, Alland D, Sacchettini JC, Jacobs WR Jr. "Mycothiol biosynthesis is essential for ethionamide susceptibility in Mycobacterium tuberculosis." Mol Microbiol. 2008 Sep;69(5):1316-29.
Dey S, Burton RL, Grant GA, Sacchettini JC. "Structural analysis of substrate and effector binding in Mycobacterium tuberculosis D-3-phosphoglycerate dehydrogenase." Biochemistry. 2008 Aug 12;47(32):8271-82.
Javid-Majd F, Yang D, Ioerger TR, Sacchettini JC. "The 1.25 A resolution structure of phosphoribosyl-ATP pyrophosphohydrolase from Mycobacterium tuberculosis." Acta Crystallogr D Biol Crystallogr. 2008 Jun;64(Pt 6):627-35. Epub 2008 May 14.
Mohamedmohaideen NN, Palaninathan SK, Morin PM, Williams BJ, Braunstein M, Tichy SE, Locker J, Russell DH, Jacobs WR Jr, Sacchettini JC. "Structure and function of the virulence-associated high-temperature requirement A of Mycobacterium tuberculosis." Biochemistry. 2008 Jun 10;47(23):6092-102.
Chinnaswamy S, Yarbrough I, Palaninathan S, Kumar CT, Vijayaraghavan V, Demeler B, Lemon SM, Sacchettini JC, Kao CC. "A locking mechanism regulates RNA synthesis and host protein interaction by the hepatitis C virus polymerase." J Biol Chem. 2008 Jul 18;283(29):20535-46.
Alahari A, Trivelli X, Guérardel Y, Dover LG, Besra GS, Sacchettini JC, Reynolds RC, Coxon GD, Kremer L. "Thiacetazone, an antitubercular drug that inhibits cyclopropanation of cell wall mycolic acids in mycobacteria." PLoS ONE. 2007 Dec 19;2(12):e1343.
Sacchettini JC, Rubin EJ, Freundlich JS. "Drugs versus bugs: in pursuit of the persistent predator Mycobacterium tuberculosis." Nat Rev Microbiol. 2008 Jan;6(1):41-52. Review.
Bhardwaj K, Palaninathan S, Alcantara JM, Yi LL, Guarino L, Sacchettini JC, Kao CC. "Structural and functional analyses of the severe acute respiratory syndrome coronavirus endoribonuclease Nsp15." J Biol Chem. 2008 Feb 8;283(6):3655-64.
Reddy MC, Gokulan K, Jacobs WR Jr, Ioerger TR, Sacchettini JC."Crystal structure of Mycobacterium tuberculosis LrpA, a leucine-responsive global regulator associated with starvation response." Protein Sci. 2008 Jan;17(1):159-70.
Publications 2007
Alahari A, Trivelli X, Guérardel Y, Dover LG, Besra GS, Sacchettini JC, Reynolds RC, Coxon GD, Kremer L. "Thiacetazone, an antitubercular drug that inhibits cyclopropanation of cell wall mycolic acids in mycobacteria." PLoS ONE. 2007 Dec 19;2(12):e1343.
Sacchettini JC, Rubin EJ, Freundlich JS. "Drugs versus bugs: in pursuit of the persistent predator Mycobacterium tuberculosis." Nat Rev Microbiol. 2008 Jan;6(1):41-52. Review.
Murillo AC, Li HY, Alber T, Baker EN, Berger JM, Cherney LT, Cherney MM, Cho YS, Eisenberg D, Garen CR, Goulding CW, Hung LW, Ioerger TR, Jacobs WR, James MN, Kim C, Krieger I, Lott JS, Sankaranarayanan R, Segelke BW, Terwilliger TC, Wang F, Wang S, Sacchettini JC. "High throughput crystallography of TB drug targets."
Infect Disord Drug Targets. 2007 Jun;7(2):127-39. Review.
Reddy MC, Palaninathan SK, Shetty ND, Owen JL, Watson MD, Sacchettini JC. "High resolution crystal structures of Mycobacterium tuberculosis adenosine kinase: insights into the mechanism and specificity of this novel prokaryotic enzyme." J Biol Chem. 2007 Sep 14;282(37):27334-42.
Freundlich JS, Wang F, Tsai HC, Kuo M, Shieh HM, Anderson JW, Nkrumah LJ, Valderramos JC, Yu M, Kumar TR, Valderramos SG, Jacobs WR Jr, Schiehser GA, Jacobus DP, Fidock DA, Sacchettini JC. "X-ray structural analysis of Plasmodium falciparum enoyl acyl carrier protein reductase as a pathway toward the optimization of triclosan antimalarial efficacy." J Biol Chem. 2007 Aug 31;282(35):25436-44.
Tsai CL, Gokulan K, Sobrado P, Sacchettini JC, Fitzpatrick PF. "Mechanistic and structural studies of H373Q flavocytochrome b2: effects of mutating the active site base." Biochemistry. 2007 Jul 3;46(26):7844-51.
Cai X, Lorraine Fuller A, McDougald LR, Tan X, Cai J, Wang F, Sacchettini JC, Zhu G. "Biochemical characterization of enoyl reductase involved in Type II fatty acid synthesis in the intestinal coccidium Eimeria tenella (Phylum Apicomplexa)." FEMS Microbiol Lett. 2007 Jul;272(2):238-44.
Wang F, Langley R, Gulten G, Wang L, Sacchettini JC. "Identification of a type III thioesterase reveals the function of an operon crucial for Mtb virulence." Chem Biol. 2007 May;14(5):543-51.
Nicola G, Smith CA, Lucumi E, Kuo MR, Karagyozov L, Fidock DA, Sacchettini JC, Abagyan R. "Discovery of novel inhibitors targeting enoyl-acyl carrier protein reductase in Plasmodium falciparum by structure-based virtual screening." Biochem Biophys Res Commun. 2007 Jul 6;358(3):686-91.
Dey S, Hu Z, Xu XL, Sacchettini JC, Grant GA. "The effect of hinge mutations on effector binding and domain rotation in Escherichia coli D-3-phosphoglycerate dehydrogenase." J Biol Chem. 2007 Jun 22;282(25):18418-26.
Ramesh A, Savva CG, Holzenburg A, Sacchettini JC. "Crystal structure of Rsr, an ortholog of the antigenic Ro protein, links conformational flexibility to RNA binding activity." J Biol Chem. 2007 May 18;282(20):14960-7.
Wang F, Langley R, Gulten G, Dover LG, Besra GS, Jacobs WR Jr, Sacchettini JC. "Mechanism of thioamide drug action against tuberculosis and leprosy." J Exp Med. 2007 Jan 22;204(1):73-8.
Sridharan S, Wang L, Brown AK, Dover LG, Kremer L, Besra GS, Sacchettini JC. "X-ray crystal structure of Mycobacterium tuberculosis beta-ketoacyl acyl carrier protein synthase II (mtKasB)." J Mol Biol. 2007 Feb 16;366(2):469-80.
Liu T, Ramesh A, Ma Z, Ward SK, Zhang L, George GN, Talaat AM, Sacchettini JC, Giedroc DP. "CsoR is a novel Mycobacterium tuberculosis copper-sensing transcriptional regulator." Nat Chem Biol. 2007 Jan;3(1):60-8.
Sidhu AB, Sun Q, Nkrumah LJ, Dunne MW, Sacchettini JC, Fidock DA. "In vitro efficacy, resistance selection, and structural modeling studies implicate the malarial parasite apicoplast as the target of azithromycin." J Biol Chem. 2007 Jan 26;282(4):2494-504.
Publications 2006
Pai R, Sacchettini J, Ioerger T. (2006) “Identifying non-crystallographic symmetry in protein electron-density maps: a feature-based approach”. Acta Crystallogr D Biol Crystallogr. 9, 1012-1021.
Wang F, Cassidy C, Sacchettini JC. (2006) “Crystal Structure and Activity Studies of the Mycobacterium tuberculosis Beta-Lactamase Reveal its Critical Role in Beta-Lactam Antibiotics Resistance”. Antimicro. Agents Chemother. 8, 2762-2771
Gould TA, Langemheen H, Sacchettini JC. (2006) “Dual role of isocitrate lyase 1 in the glyoxylate and methylcitrate cycles in Mycobacterium tuberculosis”. Mol. Microbiol. 4, 940-947.
Vilchèze C, Wang F, Arai M, Hazbón MH, Colangeli R, Kremer L, Weisbrod TR, Alland D, Sacchettini JC, Jacobs WR, Jr. Transfer of a Point Mutation in Mycobacterium tuberculosis inhA Resolves the Target of Isoniazid. Nature Medicine, in press, 2006
Gopal K, Romo TD, McKee EW, Pai R., Smith JN, Sacchettini JC, Ioerger TR. TEXTAL: Crystallographic protein model-building using AI and pattern recognition. AI Magazine, in press, 2006
Gopal K, McKee EW, Romo TD, Pai R, Smith JN, Sacchettini JC, Ioerger TR. Crystallographic protein model-building on the web. Bioinformatics, in press, 2006
Romo TD, Gopal K, McKee EW, Kanbi L, Pai R, Smith JN, Sacchettini JC and Ioerger TR. TEXTAL: AI-based structural determination for x-ray protein crystallography. IEEE Intelligent Systems. 2006 20(6):59-63.
Zhao X, Yu H, Yu S Wang F, Sacchettini JC, Magliozzo RS. Hydrogen peroxide-mediated isoniazid activation catalyzed by Mycobacterium tuberculosis catalase-peroxide (KatG) and its S315T mutant. Biochemistry, 2006 45:4131-4140.
Gaffin RD, Gokulan K, Sacchettini JC, Hewett TE, Klevitsky R, Robbins J, Sarin V, Zawieja DC, Meininger GA, Muthuchamy M. Changes in the End-to-End Interactions of Tropomyosin Affect Mouse Cardiac Muscle Dynamics. Am J Physiol Hear Circ Physiol. 2006
Freundlich JS, Yu M, Lucumi E, Kuo M, Tsai HC, Valderramos JC, Karagyozov L, Jacobs WR Jr, Schiehser GA, Fidock DA, Jacobus DP, Sacchettini JC. Synthesis and biological activity of diaryl ether inhibitors of malarial enoyl acyl carrier protein reductase. Part 2: 2'-substituted triclosan derivatives. Bioorg Med Chem Lett. 2006 16:2163-2169.
Morbidoni HR, Vilchez C, Kremer L, Bittman R, Sacchettini JC, Jacobs WR Jr. Dual inhibition of mycobacterial fatty acid biosynthesis and degradation by 2-alkynoic acids. Chem Biol. 2006 13:297-307
Owens RM, Hsu FF, Vanderven BC, Purdy GE, Hesteande E, Giannakas P, Sacchettini JC, McKinney JD, Hill PJ, Belisle JT, Butcher BA, Pethe K, Russell DG M. tuberculosis Rv2252 encodes a diacylglycerol kinase involved in the biosynthesis of phosphatidylinositol mannosides (PIMs). Mol Microbiol. 2006 60:1152-1163.
Publications 2005
Gopal K, Romo TD, Mckee EW, Childs KC, Kanbi L, Pai R, Smith JN, Sacchettini JC and Ioerger TR. TEXTAL: Automated crystallographic protein structure determination. In: Proceedings of the Innovative Applications of Artificial Intelligence conference 2005.
Paricharttanakul NM, Ye S, Menefee AL, Javid-Majd F, Sacchettini JC, Reinhart GD. Kinetic and structural characterization of phosphofructokinase from Lactobacillus bulgaricus. Biochemistry. 2005 44:15280-15286.
Bhatt A, Kremer L, Dai AZ, Sacchettini JC, Jacobs WR Jr. Conditional depletion of KasA, a key enzyme of mycolic acid biosynthesis, leads to mycobacterial cell lysis. J Bacteriol. 2005 187:7596-7606.
McKee EW, Kanbi LD, Childs KL, Grosse-Kunstleve RW, Adams PD, Sacchettini JC, Ioerger TR. FINDMOL: automated identification of macromolecules in electron-density maps. Acta Crystallogr D Biol Crystallogr. 2005 61:1514-1520.
Freundlich JS, Anderson JW, Sarantakis D, Shieh HM, Yu M, Valderramos JC, Lucumi E, Kuo M, Jacobs WR Jr, Fidock DA, Schiehser GA, Jacobus DP, Sacchettini JC. Synthesis, biological activity, and X-ray crystal structural analysis of diaryl ether inhibitors of malarial enoyl acyl carrier protein reductase. Part 1: 4'-substituted triclosan derivatives, Bioorg Med Chem Lett.2005 15:5247-5252.
Gao T, Zhang X, Xia Y, Cho Y, Sacchettini JC, Golden SS, Liwang AC. 1H, 13C and 15N chemical shift assignments of the C-terminal, 133-residue pseudo-receiver domain of circadian input kinase (CikA) in Synechococcus elongates. J Biomol NMR.2005 32: 259.
Brown AK, Sridharan S, Kremer L, Lindenberg S, Dover LG, Sacchettini JC, Besra GS. Probing the mechanism of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthase III mtFabH: factors influencing catalysis and substrate specificity.
J Biol Chem. 2005 280:32539-32547.
Gokulan K, Khare S, Ronning DR, Linthicum SD, Sacchettini JC, Rupp B. Cocrystal structures of NC6.8 Fab identify key interactions for high potency sweetener recognition: implications for the design of synthetic sweeteners. Biochemistry, 2005 44:9889-9898.
Colangeli R, Helb D, Sridharan S, Sun J, Varma-Basil M, Hazbon MH, Harbacheuski R, Megjugorac NJ, Jacobs WR Jr, Holzenburg A, Sacchettini JC, Alland D. The Mycobacterium tuberculosis iniA gene is essential for activity of an efflux pump that confers drug tolerance to both isoniazid and ethambutol. Mol Microbiol. 2005 55:1829-1840.
Johnson SM, Petrassi HM, Palaninathan SK, Mohamedmohaideen NN, Purkey HE, Nichols C, Chiang KP, Walkup T, Sacchettini JC, Sharpless KB, Kelly JW. Bisaryloxime ethers as potent inhibitors of transthyretin amyloid fibril formation. J Med Chem. 2005 48:1576-1587.
Gopal K, Romo TD, Sacchettini JC and Ioerger TR. Determining relevant features to recognize electron density patterns in x-ray protein crystallography. Journal of Bioinformatics & Computational Biology. 2005 3(3):645-676.
Sridharan S, Razvi A, Scholtz JM, Sacchettini JC. The HPr proteins from the thermophile Bacillus stearothermophilus can form domain-swapped dimers. J Mol Biol. 2005 Feb 25;346(3):919-31. Epub 2004 Dec 23.
Vilcheze C, Weisbrod TR, Chen B, Kremer L, Hazbon MH, Wang F, Alland D, Sacchettini JC, Jacobs WR Jr. Altered NADH/NAD+ ratio mediates coresistance to isoniazid and ethionamide in mycobacteria. Antimicrob Agents Chemother. 2005 Feb;49(2):708-20.
Lee JE, Kim K, Sacchettini JC, Smith CV, Safe S. DRIP150 coactivation of estrogen receptor alpha in ZR-75 breast cancer cells is independent of LXXLL motifs. J Biol Chem. 2005 280:8819-8830.
Dey S, Grant GA, Sacchettini JC. Crystal structure of Mycobacterium tuberculosis D-3-phosphoglycerate dehydrogenase: extreme asymmetry in a tetramer of identical subunits. J Biol Chem. 2005 280:14892-14899.
Dey S, Hu Z, Xu XL, Sacchettini JC, Grant GA. D-3-Phosphoglycerate dehydrogenase from Mycobacterium tuberculosis is a link between the Escherichia coli and mammalian enzymes., J Biol Chem. 2005 280:14884-14891
Xu M, Arulandu A, Struck DK, Swanson S, Sacchettini JC, Young R. Disulfide isomerization after membrane release of its SAR domain activates P1 lysozyme. Science. 2005 Jan 7;307(5706):113-7.
Publications 2004
Gopal K, Romo TD, Sacchettini JC and Ioerger TR. Efficient retrieval of electron density patterns for modeling proteins by x-ray crystallography. In: Proceedings of the International Conference on Machine Learning and Applications 2004.
Purkey HE, Palaninathan SK, Kent KC, Smith C, Safe SH, Sacchettini JC, Kelly JW. Hydroxylated polychlorinated biphenyls selectively bind transthyretin in blood and inhibit amyloidogenesis: rationalizing rodent PCB toxicity. Chem Biol. 2004 Dec;11(12):1719-28.
Gopal, K., Romo, T.D., Sacchettini, J.C., and Ioerger, T.R. (2004). Weighting features to recognize 3D patterns of electron density in X-ray protein crystallography. In: Proceedings of the Computational Systems Bioinformatics 2004.
Gopal, K., Romo, T.D., Sacchettini, J.C., and Ioerger, T.R. (2004). Evaluation of geometric and probabilistic distance measures to retrieve electron density patterns for protein structure determination. In: Proceedings of the International Conference on Artificial Intelligence.
Ioerger, T. R. and Sacchettini, J. C. (2004) TEXTAL system: artificial intelligence techniques for automated protein model building. Methods in Enzymology 374, 244-270.
Ronning DR, Vissa V, Besra GS, Belisle JT, Sacchettini JC. Mycobacterium tuberculosis antigen 85A and 85C structures confirm binding orientation and conserved substrate specificity. J Biol Chem. 2004 Aug 27;279(35):36771-7. Epub 2004 Jun 10.
Ye S, Vakonakis I, Ioerger TR, LiWang AC, Sacchettini JC. Crystal structure of circadian clock protein KaiA from Synechococcus elongatus. J Biol Chem. 2004 May 7;279(19):20511-8. Epub 2004 Mar 08.
Gaffin RD, Gokulan K, Sacchettini JC, Hewett T, Klevitsky R, Robbins J, Muthuchamy M. Charged residue changes in the carboxy-terminus of alpha-tropomyosin alter mouse cardiac muscle contractility. J Physiol. 2004 Apr 15;556(Pt 2):531-43. Epub 2004 Feb 06.
Adamski-Werner SL, Palaninathan SK, Sacchettini JC, Kelly JW. Diflunisal analogues stabilize the native state of transthyretin. Potent inhibition of amyloidogenesis. J Med Chem. 2004 Jan 15;47(2):355-74.
Adams PD, Gopal K, Grosse-Kunstleve RW, Hung LW, Ioerger TR, McCoy AJ, Moriarty NW, Pai RK, Read RJ, Romo TD, Sacchettini JC, Sauter NK, Storoni LC, Terwilliger TC. Recent developments in the PHENIX software for automated crystallographic structure determination. J Synchrotron Radiat. 2004 Jan 1;11(Pt 1):53-5.
Smith CV, Sharma V, Sacchettini JC. TB drug discovery: addressing issues of persistence and resistance. Tuberculosis (Edinb). 2004;84(1-2):45-55. Review.
Publications 2003
Green NS, Palaninathan SK, Sacchettini JC, Kelly JW. Synthesis and characterization of potent bivalent amyloidosis inhibitors that bind prior to transthyretin tetramerization. J Am Chem Soc., 2003 125, 13404-13414.
Gopal, K., Pai, R., Ioerger, T.R., Romo, T.D., and Sacchettini, J.C. (2003). TEXTAL: Artificial intelligence techniques for automated protein structure determination. In: Proceedings of the Fifteenth Conference on Innovative Applications of Artificial Intelligence Conference
Adams, P. D., Grosse-Kunstleve, R. W., Hung, L. W., Ioerger, T. R., McCoy, A. J., Moriarty, N. W., Read, R. J., Sacchettini, J. C., Sauter, N. K., Terwilliger, T. C. (2002) PHENIX: building new software for automated crystallographic structure determination. Acta Crystallographica D58, 1948-1954.
Adams, P. D., Gopal, K., Grosse-Kunstleve, R. W., Hung, L. W., Ioerger, T. R., McCoy, A. J., Moriarty, N. W., Pai, R. K., Read, R. J., Romo, T. D., Sacchettini, J. C., Sauter, N. K., Storoni, L. C., Terwilliger, T. C. (2003) Recent developments in the PHENIX software for automated crystallographic structure determination. Journal of Synchrotron Radiation 11, 53-55
Reddy V, Swanson SM, Segelke B, Kantardjieff KA, Sacchettini JC, Rupp B. Effective electron-density map improvement and structure validation on a Linux multi-CPU web cluster: The TB Structural Genomics Consortium Bias Removal Web Service. Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2200-10.
Smith CV, Sacchettini JC. Mycobacterium tuberculosis: a model system for structural genomics. Curr Opin Struct Biol. 2003 Dec;13(6):658-64. Review.
Green NS, Palaninathan SK, Sacchettini JC, Kelly JW. Synthesis and characterization of potent bivalent amyloidosis inhibitors that bind prior to transthyretin tetramerization. J Am Chem Soc. 2003 Nov 5;125(44):13404-14.
Eicken C, Pennella MA, Chen X, Koshlap KM, VanZile ML, Sacchettini JC, Giedroc DP. A metal-ligand-mediated intersubunit allosteric switch in related SmtB/ArsR zinc sensor proteins. J Mol Biol. 2003 Oct 31;333(4):683-95.
Takano K, Scholtz JM, Sacchettini JC, Pace CN. The contribution of polar group burial to protein stability is strongly context-dependent. J Biol Chem. 2003 Aug 22;278(34):31790-5. Epub 2003 Jun 10.
Zhang F, Lucke C, Baier LJ, Sacchettini JC, Hamilton JA. Solution structure of human intestinal fatty acid binding protein with a naturally-occurring single amino acid substitution (A54T) that is associated with altered lipid metabolism. Biochemistry. 2003 Jun 24;42(24):7339-47.
Razavi H, Palaninathan SK, Powers ET, Wiseman RL, Purkey HE, Mohamedmohaideen NN, Deechongkit S, Chiang KP, Dendle MT, Sacchettini JC, Kelly JW. Benzoxazoles as transthyretin amyloid fibril inhibitors: synthesis, evaluation, and mechanism of action. Angew Chem Int Ed Engl. 2003 Jun 23;42(24):2758-61.
Kremer L, Dover LG, Morbidoni HR, Vilcheze C, Maughan WN, Baulard A, Tu SC, Honore N, Deretic V, Sacchettini JC, Locht C, Jacobs WR Jr, Besra GS. Inhibition of InhA activity, but not KasA activity, induces formation of a KasA-containing complex in mycobacteria. J Biol Chem. 2003 Jun 6;278(23):20547-54. Epub 2003 Mar 24.
Kuo MR, Morbidoni HR, Alland D, Sneddon SF, Gourlie BB, Staveski MM, Leonard M, Gregory JS, Janjigian AD, Yee C, Musser JM, Kreiswirth B, Iwamoto H, Perozzo R, Jacobs WR Jr, Sacchettini JC, Fidock DA. Targeting tuberculosis and malaria through inhibition of Enoyl reductase: compound activity and structural data. J Biol Chem. 2003 Jun 6;278(23):20851-9. Epub 2003 Feb 26.
Gokulan K, Rupp B, Pavelka MS Jr, Jacobs WR Jr, Sacchettini JC. Crystal structure of Mycobacterium tuberculosis diaminopimelate decarboxylase, an essential enzyme in bacterial lysine biosynthesis. J Biol Chem. 2003 May 16;278(20):18588-96. Epub 2003 Mar 10.
Cho Y, Sharma V, Sacchettini JC. Crystal structure of ATP phosphoribosyltransferase from Mycobacterium tuberculosis. J Biol Chem. 2003 Mar 7;278(10):8333-9. Epub 2003 Jan 02.
Sharma V, Arockiasamy A, Ronning DR, Savva CG, Holzenburg A, Braunstein M, Jacobs WR Jr, Sacchettini JC. Crystal structure of Mycobacterium tuberculosis SecA, a preprotein translocating ATPase. Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2243-8. Epub 2003 Feb 26.
Smith CV, Huang CC, Miczak A, Russell DG, Sacchettini JC, Honer zu Bentrup K. Biochemical and structural studies of malate synthase from Mycobacterium tuberculosis. J Biol Chem. 2003 Jan 17;278(3):1735-43. Epub 2002 Oct 21.
Ioerger TR, Sacchettini JC. TEXTAL system: artificial intelligence techniques for automated protein model building. Methods Enzymol. 2003;374:244-70.
Terwilliger TC, Park MS, Waldo GS, Berendzen J, Hung LW, Kim CY, Smith CV, Sacchettini JC, Bellinzoni M, Bossi R, De Rossi E, Mattevi A, Milano A, Riccardi G, Rizzi M, Roberts MM, Coker AR, Fossati G, Mascagni P, Coates AR, Wood SP, Goulding CW, Apostol MI, Anderson DH, Gill HS, Eisenberg DS, Taneja B, Mande S, Pohl E, Lamzin V, Tucker P, Wilmanns M, Colovos C, Meyer-Klaucke W, Munro AW, McLean KJ, Marshall KR, Leys D, Yang JK, Yoon HJ, Lee BI, Lee MG, Kwak JE, Han BW, Lee JY, Baek SH, Suh SW, Komen MM, Arcus VL, Baker EN, Lott JS, Jacobs W Jr, Alber T, Rupp B. The TB structural genomics consortium: a resource for Mycobacterium tuberculosis biology. Tuberculosis (Edinb). 2003;83(4):223-49. Review.
Publications 2002
Tao, Y., Ioerger, T.R., and Sacchettini, J.C. (2002). Extracting Fractal Features for Analyzing Protein Structure. In: Proceedings of the IEEE Conference on Pattern Recognition
Ioerger, T.R. and Sacchettini, J.C. (2002). Automatic Modeling of Protein Backbones in Electron Density Maps via Prediction of C-alpha Coordinates. Acta Crystallographica, D58(12):2043-2054.
Adams PD, Grosse-Kunstleve RW, Hung LW, Ioerger TR, McCoy AJ, Moriarty NW, Read RJ, Sacchettini JC, Sauter NK, Terwilliger TC. PHENIX: building new software for automated crystallographic structure determination. Acta Crystallogr D Biol Crystallogr. 2002 Nov;58(Pt 11):1948-54.
Riley-Lovingshimer MR, Ronning DR, Sacchettini JC, Reinhart GD. Reversible ligand-induced dissociation of a tryptophan-shift mutant of phosphofructokinase from Bacillus stearothermophilus. Biochemistry. 2002 Oct 29;41(43):12967-74.
Larsen MH, Vilcheze C, Kremer L, Besra GS, Parsons L, Salfinger M, Heifets L, Hazbon MH, Alland D, Sacchettini JC, Jacobs WR Jr. Overexpression of inhA, but not kasA, confers resistance to isoniazid and ethionamide in Mycobacterium smegmatis, M. bovis BCG and M. tuberculosis.Mol Microbiol. 2002 Oct;46(2):453-66.
Eicken C, Sharma V, Klabunde T, Lawrenz MB, Hardham JM, Norris SJ, Sacchettini JC. Crystal structure of Lyme disease variable surface antigen VlsE of Borrelia burgdorferi. J Biol Chem. 2002 Jun 14;277(24):21691-6. Epub 2002 Mar 28.
Goulding CW, Apostol M, Anderson DH, Gill HS, Smith CV, Kuo MR, Yang JK, Waldo GS, Suh SW, Chauhan R, Kale A, Bachhawat N, Mande SC, Johnston JM, Lott JS, Baker EN, Arcus VL, Leys D, McLean KJ, Munro AW, Berendzen J, Sharma V, Park MS, Eisenberg D, Sacchettini J, Alber T, Rupp B, Jacobs W Jr, Terwilliger TC. The TB structural genomics consortium: providing a structural foundation for drug discovery.
Curr Drug Targets Infect Disord. 2002 Jun;2(2):121-41. Review.
Perozzo R, Kuo M, Sidhu AS, Valiyaveettil JT, Bittman R, Jacobs WR Jr, Fidock DA, Sacchettini JC. Structural elucidation of the specificity of the antibacterial agent triclosan for malarial enoyl acyl carrier protein reductase. J Biol Chem. 2002 Apr 12;277(15):13106-14. Epub 2002 Jan 15.
Sacchettini JC, Kelly JW. Therapeutic strategies for human amyloid diseases. Nat Rev Drug Discov. 2002 Apr;1(4):267-75. Review.
Huang CC, Smith CV, Glickman MS, Jacobs WR Jr, Sacchettini JC. Crystal structures of mycolic acid cyclopropane synthases from Mycobacterium tuberculosis. J Biol Chem. 2002 Mar 29;277(13):11559-69. Epub 2001 Dec 26.
Yang D, Shipman LW, Roessner CA, Scott AI, Sacchettini JC. Structure of the Methanococcus jannaschii mevalonate kinase, a member of the GHMP kinase superfamily. J Biol Chem. 2002 Mar 15;277(11):9462-7. Epub 2001 Dec 19.
Publications 2001
Holmes JH 4th, Lieberman JM, Probert CB, Marks WH, Hill ME, Paull DL, Guyton SW, Sacchettini J, Hall RA. Elevated intestinal fatty acid binding protein and gastrointestinal complications following cardiopulmonary bypass: a preliminary analysis. J Surg Res. 2001 Oct;100(2):192-6.
Jiang X, Smith CS, Petrassi HM, Hammarstrom P, White JT, Sacchettini JC, Kelly JW. An engineered transthyretin monomer that is nonamyloidogenic, unless it is partially denatured. Biochemistry. 2001 Sep 25;40(38):11442-52.
Shipman LW, Li D, Roessner CA, Scott AI, Sacchettini JC. Crystal structure of precorrin-8x methyl mutase. Structure (Camb). 2001 Jul 3;9(7):587-96.
Eicken C, Sharma V, Klabunde T, Owens RT, Pikas DS, Hook M, Sacchettini JC Crystal structure of Lyme disease antigen outer surface protein C from Borrelia burgdorferi. J Biol Chem. 2001 Mar 30;276(13):10010-5. Epub 2001 Jan 03.
Thomson CT, Kalergis AM, Sacchettini JC, Nathenson SG. A structural difference limited to one residue of the antigenic peptide can profoundly alter the biological outcome of the TCR-peptide/MHC class I interaction. J Immunol. 2001 Mar 15;166(6):3994-7.
Sacchettini JC, Baum LG, Brewer CF. Multivalent protein-carbohydrate interactions. A new paradigm for supermolecular assembly and signal transduction. Biochemistry. 2001 Mar 13;40(10):3009-15. Review.
Publications 2000
Duncan, K. and Sacchettini, J. C. Approaches to tuberculosis drug development. In Molecular Genetics of Mycobacteria (eds Hatfull, G. F. and Jacobs, Jr., W. R.) 297-307 (American Society of Microbiology, Washington, D. C., 2000).
Petrassi, H. M., Klabunde, T., Sacchettini, J. C. and Kelly, J. W. (2000) Structure-based design of N-phenyl phenoxazine transthyretin amyloid fibril inhibitors. J. Am. Chem. Soc. 122, 2178-2192.
Wolfrum C, Borchers T, Sacchettini JC, Spener F. Binding of fatty acids and peroxisome proliferators to orthologous fatty acid binding proteins from human, murine, and bovine liver. Biochemistry. 2000 Nov 21;39(46):14363.
Sacchettini JC, Ronning DR. The mycobacterial antigens 85 complex - from structure to function: response. Trends Microbiol. 2000 Oct;8(10):441.
Balendiran GK, Schnutgen F, Scapin G, Borchers T, Xhong N, Lim K, Godbout R, Spener F, Sacchettini JC. Crystal structure and thermodynamic analysis of human brain fatty acid-binding protein. J Biol Chem. 2000 Sep 1;275(35):27045-54.
McKinney JD, Honer zu Bentrup K, Munoz-Elias EJ, Miczak A, Chen B, Chan WT, Swenson D, Sacchettini JC, Jacobs WR Jr, Russell DG. Persistence of Mycobacterium tuberculosis in macrophages and mice requires the glyoxylate shunt enzyme isocitrate lyase. Nature. 2000 Aug 17;406(6797):735-8.
Sharma V, Sharma S, Hoener zu Bentrup K, McKinney JD, Russell DG, Jacobs WR Jr, Sacchettini JC. Structure of isocitrate lyase, a persistence factor of Mycobacterium tuberculosis. Nat Struct Biol. 2000 Aug;7(8):663-8.
Vilcheze C, Morbidoni HR, Weisbrod TR, Iwamoto H, Kuo M, Sacchettini JC, Jacobs WR Jr. Inactivation of the inhA-encoded fatty acid synthase II (FASII) enoyl-acyl carrier protein reductase induces accumulation of the FASI end products and cell lysis of Mycobacterium smegmatis. J Bacteriol. 2000 Jul;182(14):4059-67.
Fillgrove KL, Anderson VE. Orientation of coenzyme A substrates, nicotinamide and active site functional groups in (Di)enoyl-coenzyme A reductases. Biochemistry. 2000 Jun 13;39(23):7001-11.
Peterson NA, Anderson BF, Jameson GB, Tweedie JW, Baker EN. Crystal structure and iron-binding properties of the R210K mutant of the N-lobe of human lactoferrin: implications for iron release from transferrins. Biochemistry. 2000 Jun 6;39(22):6625-33.
Holton T, Ioerger TR, Christopher JA, Sacchettini JC. Determining protein structure from electron-density maps using pattern matching. Acta Crystallogr D Biol Crystallogr. 2000 Jun;56 ( Pt 6):722-34.
Lucke C, Zhang F, Hamilton JA, Sacchettini JC, Ruterjans H. Solution structure of ileal lipid binding protein in complex with glycocholate. Eur J Biochem. 2000 May;267(10):2929-38. Erratum in: Eur J Biochem 2000 Jul;267(13):4312.
Wolfrum C, Borchers T, Sacchettini JC, Spener F. Binding of fatty acids and peroxisome proliferators to orthologous fatty acid binding proteins from human, murine, and bovine liver. Biochemistry. 2000 Feb 15;39(6):1469-74.
O'Hagan RC, Schreiber-Agus N, Chen K, David G, Engelman JA, Schwab R, Alland L, Thomson C, Ronning DR, Sacchettini JC, Meltzer P, DePinho RA. Gene-target recognition among members of the myc superfamily and implications for oncogenesis. Nat Genet. 2000 Feb;24(2):113-9.
Ronning DR, Klabunde T, Besra GS, Vissa VD, Belisle JT, Sacchettini JC. Crystal structure of the secreted form of antigen 85C reveals potential targets for mycobacterial drugs and vaccines. Nat Struct Biol. 2000 Feb;7(2):141-6
Parikh S, Moynihan DP, Xiao G, Tonge PJ. Roles of tyrosine 158 and lysine 165 in the catalytic mechanism of InhA, the enoyl-ACP reductase from Mycobacterium tuberculosis. Biochemistry. 1999 Oct 12;38(41):13623-34.
Kurokawa H, Dewan JC, Mikami B, Sacchettini JC, Hirose M. Crystal structure of hen apo-ovotransferrin. Both lobes adopt an open conformation upon loss of iron. J Biol Chem. 1999 Oct 1;274(40):28445-52.
Rozwarski DA, Vilcheze C, Sugantino M, Bittman R, Sacchettini JC. Crystal structure of the Mycobacterium tuberculosis enoyl-ACP reductase, InhA, in complex with NAD+ and a C16 fatty acyl substrate. J Biol Chem. 1999 May 28;274(22):15582-9.
Balendiran GK, Molina JA, Xu Y, Torres-Martinez J, Stevens R, Focia PJ, Eakin AE, Sacchettini JC, Craig SP 3rd. Ternary complex structure of human HGPRTase, PRPP, Mg2+, and the inhibitor HPP reveals the involvement of the flexible loop in substrate binding. Protein Sci. 1999 May;8(5):1023-31.
Lucke C, Fushman D, Ludwig C, Hamilton JA, Sacchettini JC, Ruterjans H. A comparative study of the backbone dynamics of two closely related lipid binding proteins: bovine heart fatty acid binding protein and porcine ileal lipid binding protein. Mol Cell Biochem. 1999 Feb;192(1-2):109-21.
Ioerger TR, Holton T, Christopher JA, Sacchettini JC. TEXTAL: a pattern recognition system for interpreting electron density maps. Proc Int Conf Intell Syst Mol Biol. 1999:130-7.
Publications 1998
Sharma V, Grubmeyer C, Sacchettini JC. Crystal structure of quinolinic acid phosphoribosyltransferase from Mmycobacterium tuberculosis: a potential TB drug target. Structure. 1998 Dec 15;6(12):1587-99.
Dam TK, Oscarson S, Sacchettini JC, Brewer CF. Differential solvation of "core" trimannoside complexes of the Dioclea grandiflora lectin and concanavalin A detected by primary solvent isotope effects in isothermal titration microcalorimetry. J Biol Chem. 1998 Dec 4;273(49):32826-32.
Rozwarski DA, Swami BM, Brewer CF, Sacchettini JC. Crystal structure of the lectin from Dioclea grandiflora complexed with core trimannoside of asparagine-linked carbohydrates. J Biol Chem. 1998 Dec 4;273(49):32818-25.
Dam TK, Oscarson S, Brewer CF. Thermodynamics of binding of the core trimannoside of asparagine-linked carbohydrates and deoxy analogs to Dioclea grandiflora lectin. J Biol Chem. 1998 Dec 4;273(49):32812-7.
Klabunde T, Eicken C, Sacchettini JC, Krebs B. Crystal structure of a plant catechol oxidase containing a dicopper center. Nat Struct Biol. 1998 Dec;5(12):1084-90.
Lieberman JM, Marks WH, Cohn S, Jaicks R, Woode L, Sacchettini J, Fischer B, Moller B, Burns G. Organ failure, infection, and the systemic inflammatory response syndrome are associated with elevated levels of urinary intestinal fatty acid binding protein: study of 100 consecutive patients in a surgical intensive care unit. J Trauma. 1998 Nov;45(5):900-6.
Lim K, Owens SM, Arnold L, Sacchettini JC, Linthicum DS. Crystal structure of monoclonal 6B5 Fab complexed with phencyclidine. J Biol Chem. 1998 Oct 30;273(44):28576-82.
Peterson SA, Klabunde T, Lashuel HA, Purkey H, Sacchettini JC, Kelly JW. Inhibiting transthyretin conformational changes that lead to amyloid fibril formation. Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):12956-60.
Banerjee A, Sugantino M, Sacchettini JC, Jacobs WR Jr. The mabA gene from the inhA operon of Mycobacterium tuberculosis encodes a 3-ketoacyl reductase that fails to confer isoniazid resistance. Microbiology. 1998 Oct;144 ( Pt 10):2697-704.
Steinlein LM, Ligman CM, Kessler S, Ikeda RA. Iron release is reduced by mutations of lysines 206 and 296 in recombinant N-terminal half-transferrin. Biochemistry. 1998 Sep 29;37(39):13696-703.
Degano M, Almo SC, Sacchettini JC, Schramm VL. Trypanosomal nucleoside hydrolase. A novel mechanism from the structure with a transition-state inhibitor. Biochemistry. 1998 May 5;37(18):6277-85.
Wang J, Lim K, Smolyar A, Teng M, Liu J, Tse AG, Liu J, Hussey RE, Chishti Y, Thomson CT, Sweet RM, Nathenson SG, Chang HC, Sacchettini JC, Reinherz EL. Atomic structure of an alphabeta T cell receptor (TCR) heterodimer in complex with an anti-TCR fab fragment derived from a mitogenic antibody. EMBO J. 1998 Jan 2;17(1):10-26.
Rozwarski DA, Grant GA, Barton DH, Jacobs WR Jr, Sacchettini JC. Modification of the NADH of the isoniazid target (InhA) from Mycobacterium tuberculosis. Science. 1998 Jan 2;279(5347):98-102.
Klabunde T, Sharma S, Telenti A, Jacobs WR Jr, Sacchettini JC. Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing. Nat Struct Biol. 1998 Jan;5(1):31-6.
Miesel L, Rozwarski DA, Sacchettini JC, Jacobs WR Jr. Mechanisms for isoniazid action and resistance. Novartis Found Symp. 1998;217:209-20; discussion 220-1. Review.
Publications 1997
Young AC, Valadon P, Casadevall A, Scharff MD, Sacchettini JC. The three-dimensional structures of a polysaccharide binding antibody to Cryptococcus neoformans and its complex with a peptide from a phage display library: implications for the identification of peptide mimotopes. J Mol Biol. 1997 Dec 12;274(4):622-34.
Olsen LR, Dessen A, Gupta D, Sabesan S, Sacchettini JC, Brewer CF. X-ray crystallographic studies of unique cross-linked lattices between four isomeric biantennary oligosaccharides and soybean agglutinin. Biochemistry. 1997 Dec 9;36(49):15073-80.
Balendiran GK, Solheim JC, Young AC, Hansen TH, Nathenson SG, Sacchettini JC. The three-dimensional structure of an H-2Ld-peptide complex explains the unique interaction of Ld with beta-2 microglobulin and peptide. Proc Natl Acad Sci U S A. 1997 Jun 24;94(13):6880-5.
Jardim A, Ullman B. The conserved serine-tyrosine dipeptide in Leishmania donovani hypoxanthine-guanine phosphoribosyltransferase is essential for catalytic activity. J Biol Chem. 1997 Apr 4;272(14):8967-73.
Zhang F, Lucke C, Baier LJ, Sacchettini JC, Hamilton JA. Solution structure of human intestinal fatty acid binding protein: implications for ligand entry and exit. J Biomol NMR. 1997 Apr;9(3):213-28.
Xu Y, Eads J, Sacchettini JC, Grubmeyer C. Kinetic mechanism of human hypoxanthine-guanine phosphoribosyltransferase: rapid phosphoribosyl transfer chemistry. Biochemistry. 1997 Mar 25;36(12):3700-12.
Lieberman JM, Sacchettini J, Marks C, Marks WH. Human intestinal fatty acid binding protein: report of an assay with studies in normal volunteers and intestinal ischemia. Surgery. 1997 Mar;121(3):335-42.
Eads JC, Ozturk D, Wexler TB, Grubmeyer C, Sacchettini JC. A new function for a common fold: the crystal structure of quinolinic acid phosphoribosyltransferase. Structure. 1997 Jan 15;5(1):47-58.
Publications 1996
Quémard, A., Dessen, A., Sugantino, M., Jacobs Jr., W.R., Sacchettini, J.C. and Blanchard, J.S. (1996) Binding of catalase-peroxidase-activated isoniazid to wild-type and mutant Mycobacterium tuberculosis enoyl-ACP reductases. J. Am. Chem. Soc., 118, 1561-1562.
Liu J, Tse AG, Chang HC, Liu J, Wang J, Hussey RE, Chishti Y, Rheinhold B, Spoerl R, Nathenson SG, Sacchettini JC, Reinherz EL. Crystallization of a deglycosylated T cell receptor (TCR) complexed with an anti-TCR Fab fragment. J Biol Chem. 1996 Dec 27;271(52):33639-46.
Tarshis LC, Proteau PJ, Kellogg BA, Sacchettini JC, Poulter CD. Regulation of product chain length by isoprenyl diphosphate synthases. Proc Natl Acad Sci U S A. 1996 Dec 24;93(26):15018-23.
Nadeau OW, Falick AM, Woodworth RC. Structural evidence for an anion-directing track in the hen ovotransferrin N-lobe: implications for transferrin synergistic anion binding. Biochemistry. 1996 Nov 12;35(45):14294-303.
Irigoyen M, Kowal C, Young AC, Sacchettini JC, Diamond B. Molecular mapping of the 8.12 SLE-associated idiotype specificity at the single amino acid level. Mol Immunol. 1996 Nov;33(16):1255-65.
Scapin G, Reddy SG, Blanchard JS. Three-dimensional structure of meso-diaminopimelic acid dehydrogenase from Corynebacterium glutamicum. Biochemistry. 1996 Oct 22;35(42):13540-51.
Wheeler PR, Anderson PM. Determination of the primary target for isoniazid in mycobacterial mycolic acid biosynthesis with Mycobacterium aurum A+. Biochem J. 1996 Sep 1;318 ( Pt 2):451-7.
Lucke C, Zhang F, Ruterjans H, Hamilton JA, Sacchettini JC. Flexibility is a likely determinant of binding specificity in the case of ileal lipid binding protein. Structure. 1996 Jul 15;4(7):785-800.
Degano M, Gopaul DN, Scapin G, Schramm VL, Sacchettini JC. Three-dimensional structure of the inosine-uridine nucleoside N-ribohydrolase from Crithidia fasciculata. Biochemistry. 1996 May 14;35(19):5971-81.
Gopaul DN, Meyer SL, Degano M, Sacchettini JC, Schramm VL. Inosine-uridine nucleoside hydrolase from Crithidia fasciculata. Genetic characterization, crystallization, and identification of histidine 241 as a catalytic site residue. Biochemistry. 1996 May 14;35(19):5963-70.
Baier LJ, Bogardus C, Sacchettini JC. A polymorphism in the human intestinal fatty acid binding protein alters fatty acid transport across Caco-2 cells.J Biol Chem. 1996 May 3;271(18):10892-6.
Sacchettini JC, Blanchard JS. The structure and function of the isoniazid target in M. tuberculosis. Res Microbiol. 1996 Jan-Feb;147(1-2):36-43. Review.
Publications 1995
Papadopoulous, N.J., Sacchettini, J.C., Nathenson, S.G. and Angeletti, R. Hogue (1995) Rapid in vitro assembly of class I major histocompatibility complex. Techniques in Protein Chemistry Vol. VI, 375-383.
Sacchettini, J.C. and Blanchard, J.S. (1995) The structure and function of the isoniazid target in M. tuberculosis. In: Solving the dilemma of antimycobacterial chemotherapy. 13th Forum in Microbiology, Research Microbiol., 147, 36-42.
Hodsdon ME, Toner JJ, Cistola DP. 1H, 13C and 15N assignments and chemical shift-derived secondary structure of intestinal fatty acid-binding protein. J Biomol NMR. 1995 Sep;6(2):198-210.
Ozturk DH, Dorfman RH, Scapin G, Sacchettini JC, Grubmeyer C. Structure and function of Salmonella typhimurium orotate phosphoribosyltransferase: protein complementation reveals shared active sites. Biochemistry. 1995 Aug 29;34(34):10764-70.
Ozturk DH, Dorfman RH, Scapin G, Sacchettini JC, Grubmeyer C. Locations and functional roles of conserved lysine residues in Salmonella typhimurium orotate phosphoribosyltransferase. Biochemistry. 1995 Aug 29;34(34):10755-63.
Scapin G, Ozturk DH, Grubmeyer C, Sacchettini JC. The crystal structure of the orotate phosphoribosyltransferase complexed with orotate and alpha-D-5-phosphoribosyl-1-pyrophosphate. Biochemistry. 1995 Aug 29;34(34):10744-54.
Quemard A, Sacchettini JC, Dessen A, Vilcheze C, Bittman R, Jacobs WR Jr, Blanchard JS. Enzymatic characterization of the target for isoniazid in Mycobacterium tuberculosis. Biochemistry. 1995 Jul 4;34(26):8235-41.
Dessen A, Gupta D, Sabesan S, Brewer CF, Sacchettini JC. X-ray crystal structure of the soybean agglutinin cross-linked with a biantennary analog of the blood group I carbohydrate antigen. Biochemistry. 1995 Apr 18;34(15):4933-42.
Scapin G, Blanchard JS, Sacchettini JC. Three-dimensional structure of Escherichia coli dihydrodipicolinate reductase. Biochemistry. 1995 Mar 21;34(11):3502-12.
Reddy SG, Sacchettini JC, Blanchard JS. Expression, purification, and characterization of Escherichia coli dihydrodipicolinate reductase. Biochemistry. 1995 Mar 21;34(11):3492-501.
Dessen A, Quemard A, Blanchard JS, Jacobs WR Jr, Sacchettini JC. Crystal structure and function of the isoniazid target of Mycobacterium tuberculosis. Science. 1995 Mar 17;267(5204):1638-41.
Baier LJ, Sacchettini JC, Knowler WC, Eads J, Paolisso G, Tataranni PA, Mochizuki H, Bennett PH, Bogardus C, Prochazka M. An amino acid substitution in the human intestinal fatty acid binding protein is associated with increased fatty acid binding, increased fat oxidation, and insulin resistance. J Clin Invest. 1995 Mar;95(3):1281-7.
Young AC, Zhang W, Sacchettini JC, Nathenson SG. MHC class I-peptide interactions and TCR recognition. Cancer Surv. 1995;22:17-36. Review.
Young AC, Nathenson SG, Sacchettini JC. Structural studies of class I major histocompatibility complex proteins: insights into antigen presentation. FASEB J. 1995 Jan;9(1):26-36. Review.
Publications 1994
Chang HC, Bao Z, Yao Y, Tse AG, Goyarts EC, Madsen M, Kawasaki E, Brauer PP, Sacchettini JC, Nathenson SG, et al. A general method for facilitating heterodimeric pairing between two proteins: application to expression of alpha and beta T-cell receptor extracellular segments. Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11408-12.
Dewan JC, Grant GA, Sacchettini JC. Crystal structure of kappa-bungarotoxin at 2.3-A resolution. Biochemistry. 1994 Nov 8;33(44):13147-54.
Schreiber-Agus N, Chin L, Chen K, Torres R, Thomson CT, Sacchettini JC, DePinho RA. Evolutionary relationships and functional conservation among vertebrate Max-associated proteins: the zebra fish homolog of Mxi1. Oncogene. 1994 Nov;9(11):3167-77.
Tarshis LC, Yan M, Poulter CD, Sacchettini JC. Crystal structure of recombinant farnesyl diphosphate synthase at 2.6-A resolution. Biochemistry. 1994 Sep 13;33(36):10871-7.
Eads JC, Scapin G, Xu Y, Grubmeyer C, Sacchettini JC. The crystal structure of human hypoxanthine-guanine phosphoribosyltransferase with bound GMP. Cell. 1994 Jul 29;78(2):325-34.
Mikami B, Degano M, Hehre EJ, Sacchettini JC. Crystal structures of soybean beta-amylase reacted with beta-maltose and maltal: active site components and their apparent roles in catalysis. Biochemistry. 1994 Jun 28;33(25):7779-87.
Young AC, Scapin G, Kromminga A, Patel SB, Veerkamp JH, Sacchettini JC. Structural studies on human muscle fatty acid binding protein at 1.4 A resolution: binding interactions with three C18 fatty acids. Structure. 1994 Jun 15;2(6):523-34.
Maatman RG, Degano M, Van Moerkerk HT, Van Marrewijk WJ, Van der Horst DJ, Sacchettini JC, Veerkamp JH. Primary structure and binding characteristics of locust and human muscle fatty-acid-binding proteins. Eur J Biochem. 1994 Apr 15;221(2):801-10.
Scapin G, Grubmeyer C, Sacchettini JC. Crystal structure of orotate phosphoribosyltransferase. Biochemistry. 1994 Feb 15;33(6):1287-94.
Young AC, Zhang W, Sacchettini JC, Nathenson SG. The three-dimensional structure of H-2Db at 2.4 A resolution: implications for antigen-determinant selection. Cell. 1994 Jan 14;76(1):39-50.
Publications 1993
Eads J, Sacchettini JC, Kromminga A, Gordon JI. Escherichia coli-derived rat intestinal fatty acid binding protein with bound myristate at 1.5 A resolution and I-FABPArg106-->Gln with bound oleate at 1.74 A resolution. J Biol Chem. 1993 Dec 15;268(35):26375-85.
Dewan JC, Mikami B, Hirose M, Sacchettini JC. Structural evidence for a pH-sensitive dilysine trigger in the hen ovotransferrin N-lobe: implications for transferrin iron release. Biochemistry. 1993 Nov 16;32(45):11963-8.
Grubmeyer C, Segura E, Dorfman R. Active site lysines in orotate phosphoribosyltransferase. J Biol Chem. 1993 Sep 25;268(27):20299-304.
Sacchettini JC, Gordon JI. Rat intestinal fatty acid binding protein. A model system for analyzing the forces that can bind fatty acids to proteins. J Biol Chem. 1993 Sep 5;268(25):18399-402. Review.
Mikami B, Hehre EJ, Sato M, Katsube Y, Hirose M, Morita Y, Sacchettini JC. The 2.0-A resolution structure of soybean beta-amylase complexed with alpha-cyclodextrin.
Biochemistry. 1993 Jul 13;32(27):6836-45.
Scapin G, Young AC, Kromminga A, Veerkamp JH, Gordon JI, Sacchettini JC. High resolution X-ray studies of mammalian intestinal and muscle fatty acid-binding proteins provide an opportunity for defining the chemical nature of fatty acid: protein interactions. Mol Cell Biochem. 1993 Jun 9-23;123(1-2):3-13.
Scapin G, Sacchettini JC, Dessen A, Bhatia M, Grubmeyer C. Primary structure and crystallization of orotate phosphoribosyltransferase from Salmonella typhimurium. J Mol Biol. 1993 Apr 20;230(4):1304-8.
Publications 1992
Sacchettini JC, Scapin G, Gopaul D, Gordon JI. Refinement of the structure of Escherichia coli-derived rat intestinal fatty acid binding protein with bound oleate to 1.75-A resolution. Correlation with the structures of the apoprotein and the protein with bound palmitate. J Biol Chem. 1992 Nov 25;267(33):23534-45.
Zhang W, Young AC, Imarai M, Nathenson SG, Sacchettini JC. Crystal structure of the major histocompatibility complex class I H-2Kb molecule containing a single viral peptide: implications for peptide binding and T-cell receptor recognition. Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):8403-7.
Scapin G, Gordon JI, Sacchettini JC. Refinement of the structure of recombinant rat intestinal fatty acid-binding apoprotein at 1.2-A resolution. J Biol Chem. 1992 Feb 25;267(6):4253-69.
Green RP, Cohn SM, Sacchettini JC, Jackson KE, Gordon JI. The mouse intestinal fatty acid binding protein gene: nucleotide sequence, pattern of developmental and regional expression, and proposed structure of its protein product. DNA Cell Biol. 1992 Jan-Feb;11(1):31-41.
Publications 1991
Gordon, J.I., Sacchettini, J.C., Ropson, I.J., Frieden, C., Li, E., Rubin, D.C., Roth, K.A. and Cistola, D.P. (1991) Intracellular fatty-acid-binding proteins and their genes: Useful models for diverse biological questions. Current Opinions in Lipidology, 2, 125-137.
Publications 1990
Sacchettini JC, Hauft SM, Van Camp SL, Cistola DP, Gordon JI. Developmental and structural studies of an intracellular lipid binding protein expressed in the ileal epithelium. J Biol Chem. 1990 Nov 5;265(31):19199-207.
Sacchettini JC, Banaszak LJ, Gordon JI. Expression of rat intestinal fatty acid binding protein in E. coli and its subsequent structural analysis: a model system for studying the molecular details of fatty acid-protein interaction. Mol Cell Biochem. 1990 Oct 15-Nov 8;98(1-2):81-93.
Cistola DP, Sacchettini JC, Gordon JI. 13C NMR studies of fatty acid-protein interactions: comparison of homologous fatty acid-binding proteins produced in the intestinal epithelium. Mol Cell Biochem. 1990 Oct 15-Nov 8;98(1-2):101-10.
Publications 1989
Gantz I, Nothwehr SF, Lucey M, Sacchettini JC, DelValle J, Banaszak LJ, Naud M, Gordon JI, Yamada T. Gastrotropin: not an enterooxyntin but a member of a family of cytoplasmic hydrophobic ligand binding proteins. J Biol Chem. 1989 Dec 5;264(34):20248-54.
Li E, Quian SJ, Nader L, Yang NC, d'Avignon A, Sacchettini JC, Gordon JI. Nuclear magnetic resonance studies of 6-fluorotryptophan-substituted rat cellular retinol-binding protein II produced in Escherichia coli. Analysis of the apoprotein and the holoprotein containing bound all-trans-retinol and all-trans-retinal. J Biol Chem. 1989 Oct 15;264(29):17041-8.
Sacchettini JC, Gordon JI, Banaszak LJ. Refined apoprotein structure of rat intestinal fatty acid binding protein produced in Escherichia coli. Proc Natl Acad Sci U S A. 1989 Oct;86(20):7736-40.
Sacchettini JC, Gordon JI, Banaszak LJ. Crystal structure of rat intestinal fatty-acid-binding protein. Refinement and analysis of the Escherichia coli-derived protein with bound palmitate. J Mol Biol. 1989 Jul 20;208(2):327-39.
Cistola DP, Sacchettini JC, Banaszak LJ, Walsh MT, Gordon JI. Fatty acid interactions with rat intestinal and liver fatty acid-binding proteins expressed in Escherichia coli. A comparative 13C NMR study. J Biol Chem. 1989 Feb 15;264(5):2700-10.
Publications 1988
Sacchettini JC, Frazier MW, Chiara DC, Banaszak LJ, Grant GA. Amino acid sequence of porcine heart fumarase. Biochem Biophys Res Commun. 1988 May 31;153(1):435-40.
Sacchettini JC, Gordon JI, Banaszak LJ. The structure of crystalline Escherichia coli-derived rat intestinal fatty acid-binding protein at 2.5-A resolution. J Biol Chem. 1988 Apr 25;263(12):5815-9.
Gibson BW, Yu ZG, Aberth W, Burlingame AL, Bass NM. Revision of the blocked N terminus of rat heart fatty acid-binding protein by liquid secondary ion mass spectrometry. J Biol Chem. 1988 Mar 25;263(9):4182-5.
Banaszak, L.J., Rhodes, G. and Sacchettini, J.C. (1988) Methods and instruments for determining the structure of biological macromolecules by x-ray crystallography. Bioinstrumentation, National Association of Biology Teachers.
Publications 1987
Sacchettini JC, Stockhausen D, Li E, Banaszak LJ, Gordon JI. Crystallization of rat cellular retinol binding protein II. Preliminary X-ray data obtained from the apoprotein expressed in Escherichia coli. J Biol Chem. 1987 Nov 15;262(32):15756-8.
Lowe JB, Sacchettini JC, Laposata M, McQuillan JJ, Gordon JI. Expression of rat intestinal fatty acid-binding protein in Escherichia coli. Purification and comparison of ligand binding characteristics with that of Escherichia coli-derived rat liver fatty acid-binding protein. J Biol Chem. 1987 Apr 25;262(12):5931-7.
Sacchettini JC, Meininger TA, Lowe JB, Gordon JI, Banaszak LJ. Crystallization of rat intestinal fatty acid binding protein. Preliminary X-ray data obtained from protein expressed in Escherichia coli. J Biol Chem. 1987 Apr 15;262(11):5428-30.
Publications 1986
Sacchettini JC, Meininger T, Roderick S, Banaszak LJ. Purification, crystallization, and preliminary X-ray data for porcine fumarase. J Biol Chem. 1986 Nov 15;261(32):15183-5.
Sacchettini JC, Said B, Schulz H, Gordon JI. Rat heart fatty acid-binding protein is highly homologous to the murine adipocyte 422 protein and the P2 protein of peripheral nerve myelin. J Biol Chem. 1986 Jun 25;261(18):8218-23.
Publications 1985
Welgus HG, Grant GA, Sacchettini JC, Roswit WT, Jeffrey JJ. The gelatinolytic activity of rat uterus collagenase. J Biol Chem. 1985 Nov 5;260(25):13601-6.
Publications 1984
Publications 1983
Grant GA, Sacchettini JC, Welgus HG. A collagenolytic serine protease with trypsin-like specificity from the fiddler crab Uca pugilator. Biochemistry. 1983 Jan 18;22(2):354-8.
Patents
J. Sacchettini (1999) “InhA crystals and three-dimensional structure”
J. Sacchettini, J. Blanchard & W.R. Jacobs (1998) “Method and compounds for inhibiting lipid biosynthesis of bacteria and plants”
J. Sacchettini, J. Blanchard and W.R. Jacobs, Jr. and R. Bittman (1998) “Antimycobacterial compounds and method of using same”
J. Sacchettini (1996) “Crystalline InhA enzyme-NADH complex”
J. Sacchettini & J. Blanchard (1995) “Dihydrodipicolinate reductase crystals and three dimensional structure”
J. Sacchettini (1994) “Farnesyl pyrophosphate synthase crystals and method of preparation”
